Oxygenase Activity of Microorganisms in Oil-Contaminated Soils under Photoluminescence Films
D.A. Filatov, L.I. Svarovskaya, L.K. Altunina,
Institute of Petroleum Chemistry SB RAS, Tomsk, Russia
The effect of polyethylene films exhibiting photoluminescence properties on the growth and oxygenase activity of microflora in oil-contaminated soils has been studied under the field conditions. Stimulating effect of the photoluminescence films on biocenosis of oil-contaminated soils has been studied at concentration of 5 %. At the same time the number of major groups of the aboriginal microflora increased by 2-3 orders. The increase in number was accompanied with increased oxygenase activity of the microorganisms. At the end of the experiment in 80 days the residual content of oil decreased by 96 %. Chromatographic analysis of the residual saturated hydrocarbons demonstrated, that light hydrocarbons Ñ9-Ñ14 were completely eliminated and concentration of high-molecular hydrocarbons decreased by 85-90 %. A coefficient of hydrocarbons biodestruction, determined as ratio of the sum of n-alkanes (C17+C18) to the sum of isoalkanes (pristane+phytane), increased 4-5 times.