Cojocaru A.F., Prisakaru V.I.*, Stoikova S.V.*
The institute of Cellular Biophysics RAS, Pushchino, Russia
*State University of medicine and pharmacology named by N.Testimitchan, Kishynev, Republic Moldova
Complex biophysical method of investigation and preliminary selection of biologically active substances (Cojocaru, 1992), model and native membranes were used to study the molecular mechanism of action of large group of new synthesized antiparasitosis substances from the class of substituted salicylanilides (SSA) and preparations of nitrophuran row (PNP) with bactericide, fungicide and fasciolocide action on laboratory animals under different concentrations, starting from nanomole ones (10-9 – 10-3 M). On bilayer lipid membranes (BLM) the majority of SSA and PNP was efficient protonophores, other substances were less efficient ones in this way, last ones were rather active antioxidants at the same time. It was shown that protonophore action of substances studied on BLM correlated with their uncoupling action and reducing ATP synthesis efficiency on mitochondria and with antiparasitosis action on the whole organism. The preparation activity depended on the coefficient of dissociation for different ion forms, coefficient of distribution lipid/medium, nature and quantity of substitutes in benzol rings and five member cycles.